Organ culture of mucosal biopsies of human small intestine

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Organ culture of mucosal biopsies of human small intestine.

In vitro experiments of small intestinal mucosal function and metabolism utilizing excised tissue have been limited to a few hours by rapid epithelial cell necrosis which occurs with current incubation methods. We describe a method for culturing human mucosal biopsies for up to 24 hr employing organ culture methodology and demonstrate its potential application to studies of mucosal function. Pe...

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ژورنال

عنوان ژورنال: Journal of Clinical Investigation

سال: 1969

ISSN: 0021-9738

DOI: 10.1172/jci106108